Ronaldo was shown a second yellow card for a perceived dive in the Supercopa first leg, just two minutes after he fired Madrid into a 2-1 lead, prompting Zidane to express his frustration with the referee.
Real Madrid took a 3-1 victory out of the Spanish Super Cup match.
With the second leg mere days away, the excitement is building ahead of a rematch between two great sides.
Replay showed that there was absolutely no much contact.
Under new manager, Ernesto Valverde, it will be interesting to see if the team flourishes or not, and the gaffer has a tough task, facing Real Madrid for the title.
Ronaldo rubs it in, returning the punch to Messi and Barcelona.
With just over 49 minutes gone on the clock, the Catalans gifted their rivals the opening goal as Gerard Pique could only turn Marcelo's low delivery past a helpless Ter Stegen and into the back of his own net.
However, Ronaldo could face a ban of between four and 12 games after pushing referee Ricardo De Burgos Bengoetxea as he left the field.
Barca midfielder Sergio Busquets said: "A 3-1 defeat at home is very hard to take; the tie seems very tough for us now but we'll try and make a go of it, even though we've made it nearly impossible for us".
Barca are still reeling from the loss of Neymar to Paris Saint-Germain for a world record 222 million euros ($262 million) with their attempts to land a replacement in Liverpool's Philippe Coutinho and Ousmane Dembele of Borussia Dortmund so far falling short.
The 53-year-old has plenty of experience in La Liga, having managed Espanyol, Villarreal, Valencia and Athletic Bilbao, but the pressure cooker of the Camp Nou will be an altogether new challenge.
"We've had a good pre-season and we are coming into the game in good condition".
Marco Asensio scored Madrid's final goal in the 90th minute.
"What annoys me is the sending off for Cristiano", said the Frenchman. "We're fighting for a trophy and it's an important step".