Matt Kenseth, who is looking for his third win in four New Hampshire races, was ninth fastest (29.236 seconds).
Larson's 133.324 m.p.h. fast lap, however, was thrown out following post-qualifying inspection. Busch's fastest lap of 29.086 seconds, with a speed of 130.950 miles per hour, was enough to best Martin Truex Jr. by.085 seconds. He will start at the rear of the field in Sunday's Overton's 301 race. Hamlin was in a backup auto after damaging his primary in a crash during practice earlier Friday. Larson will start last in Sunday's 301-lapper after his apparent pole-winning qualifying speed was disallowed Friday.
"Boy, that had a lot of turn", Elliott said after completing 26 laps.
Byron is in his first year of Xfinity Series racing.
NASCAR's eyes got bigger at New Hampshire - though Larson did pass pre-qualifying inspection. Truex confirmed the speed that initially put him on the outside of the front row in Friday's qualification.
Martin Truex Jr. will start from the pole. He'll start first in Sunday's Overton's 301 (3 p.m. ET, NBCSN, PRN, SiriusXM NASCAR Radio) on the 1.058-mile track.
Larson's No. 42 Chevrolet was penalized for a rear brake cooling assembly that did not meet standards. Larson had initially taken first place in Friday's qualifying, but his speed was disallowed after his vehicle failed post-qualifying inspection for an unapproved rear decklid fin.
Hamlin set the quick pace in his second lap of practice with a speed of 130.568mph in the back up vehicle.
Aric Almirola, in the midst of his first race weekend since breaking his back in a May 13 crash at Kansas, was 25th-fastest in the Richard Petty Motorsports No. 43 Ford.
The on-track traction will come from the surface at NHMS being treated with PJ1 TrackBite, a type of resin that will be applied to the very bottom of the track on the yellow lane, and two lanes above the racing groove.